American Indian adolescent girls face significant challenges concerning their mental health, often exacerbated by historical trauma and systemic inequalities born from racist and sexist settler colonial practices. These cultural factors, including the loss of cultural identity and connection to ancestral lands, can contribute to feelings of isolation and depression among American Indian adolescent girls. Moreover, the intergenerational transmission of trauma resulting from colonialism, forced assimilation policies, and the legacy of boarding schools, which included the specific targeting of girls within historical-cultural assimilation practices, given girls’ central roles in building the next generation of American Indian families and communities continues to impact their mental well-being. This mixed methods, tribally driven participatory research study aims to understand the feasibility, acceptability, and perceived effectiveness, including pathways of change of a strengths-based, intergenerational program (Girl Societies) for American Indian adolescent girls that aims to revitalize protective matriarchal cultural practices.